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Bioneer Corporation synthetic mirna oligonucleotides
Bead-based multiplex assay workflow and representative calibration curve. (A) Schematic diagram illustrating the workflow of the bead-based multiplex assay for <t>miRNA</t> quantification. (B) The calibration curves were generated for each of the nine miRNAs, depicting the relationship between concentration (pg/μL) on the x- axis and the corresponding measured signal (MFI) on the y -axis. Each curve represents the mean values from six measurements, with error bars indicating the standard deviation (SD).
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Bead-based multiplex assay workflow and representative calibration curve. (A) Schematic diagram illustrating the workflow of the bead-based multiplex assay for miRNA quantification. (B) The calibration curves were generated for each of the nine miRNAs, depicting the relationship between concentration (pg/μL) on the x- axis and the corresponding measured signal (MFI) on the y -axis. Each curve represents the mean values from six measurements, with error bars indicating the standard deviation (SD).

Journal: ACS Omega

Article Title: Validation of a Bead-Based Multiplex Assay for miRNA Quantification in Rat Liver Inflammation

doi: 10.1021/acsomega.5c09640

Figure Lengend Snippet: Bead-based multiplex assay workflow and representative calibration curve. (A) Schematic diagram illustrating the workflow of the bead-based multiplex assay for miRNA quantification. (B) The calibration curves were generated for each of the nine miRNAs, depicting the relationship between concentration (pg/μL) on the x- axis and the corresponding measured signal (MFI) on the y -axis. Each curve represents the mean values from six measurements, with error bars indicating the standard deviation (SD).

Article Snippet: Synthetic miRNA oligonucleotides (Bioneer) were used as calibration standards and QC samples.

Techniques: Multiplex Assay, Generated, Concentration Assay, Standard Deviation

Detection of target miRNAs using a bead-based multiplex assay. The expression levels of miRNAs were assessed in the livers of male and female rats in the vehicle control (VC, saline), negative control (NC, control CpG ODN 1826), and treatment (T, CpG ODN 1826 and LPS) groups ( n = 4 rats per group). Data are shown as the mean ± SD. Statistical significance is indicated as follows: *p ≤ 0.05 (VC vs T), # p ≤ 0.05 (NC vs T), and + p ≤ 0.05 (VC vs NC).

Journal: ACS Omega

Article Title: Validation of a Bead-Based Multiplex Assay for miRNA Quantification in Rat Liver Inflammation

doi: 10.1021/acsomega.5c09640

Figure Lengend Snippet: Detection of target miRNAs using a bead-based multiplex assay. The expression levels of miRNAs were assessed in the livers of male and female rats in the vehicle control (VC, saline), negative control (NC, control CpG ODN 1826), and treatment (T, CpG ODN 1826 and LPS) groups ( n = 4 rats per group). Data are shown as the mean ± SD. Statistical significance is indicated as follows: *p ≤ 0.05 (VC vs T), # p ≤ 0.05 (NC vs T), and + p ≤ 0.05 (VC vs NC).

Article Snippet: Synthetic miRNA oligonucleotides (Bioneer) were used as calibration standards and QC samples.

Techniques: Multiplex Assay, Expressing, Control, Saline, Negative Control

Comparative analysis of miRNA expression using two different technique. The expression levels of eight miRNAs obtained from the bead-based multiplex assay (left y -axis, bar graph) and RT–qPCR (right y -axis, line graph) were presented. Data are shown as the mean ± SD, Vehicle control (VC, saline), negative control (NC, control CpG ODN 1826), and treatment (T, CpG ODN 1826 and LPS) groups ( n = 4 rats per group).

Journal: ACS Omega

Article Title: Validation of a Bead-Based Multiplex Assay for miRNA Quantification in Rat Liver Inflammation

doi: 10.1021/acsomega.5c09640

Figure Lengend Snippet: Comparative analysis of miRNA expression using two different technique. The expression levels of eight miRNAs obtained from the bead-based multiplex assay (left y -axis, bar graph) and RT–qPCR (right y -axis, line graph) were presented. Data are shown as the mean ± SD, Vehicle control (VC, saline), negative control (NC, control CpG ODN 1826), and treatment (T, CpG ODN 1826 and LPS) groups ( n = 4 rats per group).

Article Snippet: Synthetic miRNA oligonucleotides (Bioneer) were used as calibration standards and QC samples.

Techniques: Expressing, Multiplex Assay, Quantitative RT-PCR, Control, Saline, Negative Control